The catalytic activity of eukaryotic DNA polymerase enzymes is approximately 2000 to 4000 nucleotides per minute. &

The ability of bacteria to divide by mitosis and thereby make clones of themselves. Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase.

Terms

In restriction endonuclease EcoR1, “E” stands for.

b. Answer: C Topic: Polymerase Chain Reaction Section: 25.8 Difficulty Level: Medium 83) The thermal cycler heats the DNA sample to: a) 90 o C; then cools down to: b) 50-60 o C; and after it is warmed to c) about 70 o C. This steps are done in order to a) a) denature the DNA sample; b) allow the primers to bind; c) denature the DNA again. Eukaryotic DNA replication requires multiple replication forks, while prokaryotic replication uses a single origin to rapidly replicate the entire genome. (a) Micro-injection method (b) Gene gun method Contains a segment of DNA from a foreign source.

A. PCR requires DNA primers to initiate synthesis of a strand complimentary in sequence to the template.

O It must polymerize DNA 5' to 3'.

O It can only add nucleotides onto an existing polymer. b.

O It uses ATP hydrolysis to fuel polymerization.

Which of the following enzyme is suitable for dephosphorylation at 5’ end of a DNA strand? c. Is created from embryonic stem cells.

O It must polymerize DNA 5' to 3'.

is the most suited enzyme for that purpose? It is important for transcription c. It is important for translation d. It is important for PCR e. None of the above (all of the statements are true) Recombinant DNA: a.

d. Generating an organism that is a genetic copy of another organism by bypassing sexual reproduce. University of Nevada, Las Vegas • CHEMISTRY 345, University of North Texas • CHEMISTRY 2380, Florida International University • CHM 2211.

o It needs a template DNA strand. 22.

b) It polymerizes the short oligonucleotide strands formed during biosynthesis. Which of the following is not a true statement comparing prokaryotic and eukaryotic DNA replication? Which of the following is not true about Taq DNA polymerase? This preview shows page 28 - 32 out of 46 pages. & nucleotides.

88) Amino acids bear the same relation to proteins as ___ do to nucleic acids. QUESTION 7 Which of the following is NOT true of DNA Polymerase? Which of the following statement is not true in case of DNA, 20. Disclaimer: Studyref offers custom essay services and instant solutions to its clients for only limited uses.

a) It generates a large number of copies of DNA.

Which of the following is not used to transfer the recombinant DNA into the host ?

DNA replication proceeds in only one direction around the bacterial chromosome.

None of the above (all are required) Which IS NOT true about complimentary base-pairing: a. The term "cloning" refers to: a. Taq polymerase is a thermostable DNA polymerase. Question 20 Which Of The Following Statements Is Not True? O It uses ATP hydrolysis to fuel polymerization.

Answer: adenine, cytosine, guanine, and thymine. It follows the New nucleotides are added to a growing DNA strand by phosphodiester bonds. c. It is associated with a sliding clamp only on the leading strand. light microscope? d. Its exonuclease activity is involved in proofreading. It follows the law of complementary bases … Privacy

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The ability of bacteria to divide by mitosis and thereby make clones of themselves. A) Replication of DNA begins with the uncoiling of the molecule. Course Hero is not sponsored or endorsed by any college or university. a. it functions at extremely high temperatures b. it adds nucleotide triphosphates to form a new DNA strand c. it requires a salt environment (buffer to work) d. It can act as an exonuclease to cut single-stranded DNA / 1 point a. d. RNA polymerase reads a template strand of DNA 5’ to 3’. B.The double stranded DNA that your are amplifying with PCR …

View desktop site, Question 7 Which of the following is NOTtrue of DNA polymerase: Solution: It uses ATP hydrolysis to fuel polymerization.

79) Which of the following statements about the polymerase chain reaction are true? Producing multiple copies of a recombinant DNA by transforming bacteria.

19. It is important for transcription c. It is important for translation d. It is important for PCR e. None of the above (all of the statements are true) Recombinant DNA: a.

87) The four bases found in RNA are: ___. 24. 3. o It needs a template DNA strand. c. During transcription of a gene, RNA polymerase reads only one strand of DNA. Explanation: DNA polymerase is an enzyme which is used to make new DNA. Question: Question 20 Which Of The Following Statements Is Not True?

e. None of the above. B. to make it sticky which enzyme would you select for homopolymer tailing?

19. 80) If you start a PCR amplification with one molecule of double stranded DNA, how many. QUESTION 7 Which of the following is NOT true of DNA Polymerase? Privacy catalyzes the formation of a phosphodiester bond. © 2003-2020 Chegg Inc. All rights reserved.

b. It is important for DNA replication b. Both eukaryotic and prokaryotic DNA polymerases build off RNA primers made by primase. a. DNA Polymerase b. dNTPs c. Helicase d. Primers e. None of the above (all are required) Which IS NOT true about complimentary base-pairing: a.

While this is so, we "the company" shall uphold all necessary SECURITY measures to protect the IDENTITY of our clients. 85) Each section of DNA that codes for a particular protein is called a ___. Which of the following is required to perform polymerase chain reaction ? (a) Automated PCR machines are called thermal cyclers (b) A thermostable DNA polymerase is required (c) Millions to billions of desired DNA copies can be produced from microgram quantities of DNA (d) All of the above.

Is defined as a mix of human and animal DNA. 81) Which of the following is not a valid application of the PCR (polymerase chain reaction)? replication, DNA polymerase adds nucleotides to form a new DNA The Lagging Strand Of DNA Is Started By An RNA Primer.

© 2003-2020 Chegg Inc. All rights reserved. In order. Terms QUESTION 8 Which of the following would result in a mutation being inherited by both of the daughter cells after one round of cell division: O A mutation in Primase that increased its error rate O A single G-to-T transversion caused by cigarette smoke O Tautomerization of a single cytosine during DNA replication O Chemically-induced deamination of a single cytosine. Is note a true statements among the given statements.

Question: What is the total magnification of each power objective on the

Which of the following statements is accurate for the PCR – polymerase chain reaction? It adds dNTPs only in the ′ direction. She wants to wear ….

DNA (deoxyribonucleic acid) polymerase is an enzyme that catalyzes the formation of a phosphodiester bond.

Answer: adenine, cytosine, guanine, and uracil. c. Producing a transgenic animal by inserting recombinant DNA into an embryo. A. DNA Polymerase Joins Nucleotides In One Direction (5' To 3') Only.

| Large amounts of DNA must be isolated from the source organism. Ther…. A. B. DNA replication produces a DNA duplex that consists of one new strand of DNA and one old strand of DNA.

The molecules that. An automated thermocycler is used to heat and cool the reaction samples. transcribe and translate these instructions are called ___. a. DNA polymerase joins nucleotides in one direction (5' to 3') only. Question: A 28-year old bride-to-be is getting married the first week of

It helps in replication to copy DNA using the single-stranded primer- either DNA or RNA. * 20. Question: Examples of Sterilization methods oven, gas, steam under pressure

b) a) denature the DNA sample; b) allow the primers to bind the templates; c) extend each strand.

82) Primers are needed for the enzyme polymerase, 83) The thermal cycler heats the DNA sample to: a) 90. a) a) denature the DNA sample; b) allow the primers to bind; c) denature the DNA again. O It must polymerize DNA 5' to 3'. by polymerase-catalyzed condensation of monomer nucleotides.

b. Contains a segment of DNA from a foreign source. Answer.

c. Is created from embryonic stem cells.

The mechanism by which the presence of arabinose (a sugar) controls the arabinose operon: a. catabolic repression b. induction c. translation d. anabolic repression fo. The DNA polymerase is an enzyme that synthesises DNA in all living organism and thus present in almost all organism on earth.



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